Shoot regeneration and root induction in brinjal by growth regulators

Author: Md. Abdul Alim, Bhabendra Kumar Biswas, Md. Hasanuzzaman, Pronay Balaand Santanu Roy

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The experiment was carried out to study the effect of genotypes and growth regulators on root induction and shoot regeneration of brinjal (Solanummelongena) genotypes. Leaf segments of three varieties of Solanum were cultured on MS medium with different concentrations and combinations of plant growth regulators. Among the tested genotypes, Protab showed highest percentage of shoot regeneration (65.67%). Early and maximum rate of regeneration was found in MS + 1 mg/L NAA (naphthalene acetic acid) + 0.1 mg/LBAP (6-benzylamino purine) for all the genotypes. The highest number of roots per shoot was counted in Protab (73.33%) on 1/2MS + 2 mg/L IBA (indole butyric acid) + 0.4 mg/L BAP. Based on the overall performance, the variety Protab appeared as the best for shoot and root formation and ultimately successful regeneration of plants.

Key words: Regeneration; Induction; Root; Brinjal; Growth regulators

DOI: http://dx.doi.org/10.3329/ralf.v2i1.23028

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